Aug 03, 2023

Public workspaceHuman FFPE Liver Pathology Assessment -- University of Minnesota Human TMC

DOI
https://dx.doi.org/10.17504/protocols.io.6qpvr45wpgmk/v1
  • Oyedele Adeyi1,
  • Andrew Nelson1,
  • Beth Thompson1,
  • Laura Niedernhofer1
  • 1University of Minnesota Medical School, Minneapolis, MN USA
  • Cellular Senescence Network (SenNet) Method Development Community
Mickayla DuFresne-To
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DOI: https://dx.doi.org/10.17504/protocols.io.6qpvr45wpgmk/v1
Protocol Citation: Oyedele Adeyi, Andrew Nelson, Beth Thompson, Laura Niedernhofer 2023. Human FFPE Liver Pathology Assessment -- University of Minnesota Human TMC. protocols.io https://dx.doi.org/10.17504/protocols.io.6qpvr45wpgmk/v1
License: This is an open access protocol distributed under the terms of the Creative Commons Attribution License,  which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
Protocol status: Working
We use this protocol and it's working
Created: April 25, 2023
Last Modified: August 03, 2023
Protocol Integer ID: 81034
Keywords: Pathology, Liver, Human, Sample evaluation, UMN, UMinnesota, University of Minnesota, Niedernhofer, Tissue, FFPE, human ffpe liver pathology assessment, ffpe human liver sample, human liver sample, liver, minnesota human tmc purpose, university of minnesota human tmc purpose, ffpe, university of minnesota human tmc, minnesota human tmc
Funders Acknowledgements:
NIH
Grant ID: 1U54AG07041-01
Abstract
Purpose: Describe the parameters evaluated per FFPE human liver sample received/obtained for the University of Minnesota SenNet TMC. These parameters are used to evaluate whether the tissue can be characterized as "normal" vs "diseased" prior to further protocols.


Troubleshooting
Prepare two slides with serial sections of the FFPE liver sample -- 1 section per slide.
Section A -- Stain with H&E.
Section B -- Stain with trichrome.
Mount coverslip.
Schedule evaluation with pathologist (ideally with a strong liver background).
Request assessment and record of the following scores:
1. Steatosis, macro %
2. Steatosis, score (0, 1, 2, 3)
3. Ballooning (0, 1, 2)
4. Inflammation (0, 1, 2, 3)
5. Fibrosis (0, 1a, 1b, 1c, 2, 3, 4)
6. Any comments on preservation/quality of the sample
7. Other/Additional identifications (e.g. the presence of NASH or other disease states)
Record these values and consider them prior to moving the sample forward for experimentation processing.
Inventory and archive H&E and trichrome stains at room temperature -- Can be scanned if future images are required.

Inventory and archive FFPE blocks at room temperature.