Oct 13, 2023
Human Blood Sample Collection Protocol --University of Minnesota TMCs
- Laura J Niedernhofer1,
- David A Bernlohr1
- 1University of Minnesota, Minneapolis, MN USA
- Cellular Senescence Network (SenNet) Method Development Community
Protocol Citation: Laura J Niedernhofer, David A Bernlohr 2023. Human Blood Sample Collection Protocol --University of Minnesota TMCs. protocols.io https://dx.doi.org/10.17504/protocols.io.n2bvj327nlk5/v1
License: This is an open access protocol distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
Protocol status: Working
We use this protocol and it's working
Created: October 13, 2023
Last Modified: October 13, 2023
Protocol Integer ID: 89215
Keywords: attached bionet specimen procurement agreement, bionet specimen procurement agreement, university of minnesota tmcs collection protocol, minnesota tmcs collection protocol, umn ctsi biorepository, specimen procurement, sample collection, blood, laboratory, laboratory service, sample, minnesota tmc, specimen
Funders Acknowledgements:
NIH
Grant ID: 5U54AG079754-02
NIH
Grant ID: 5U54AG076041-03
Abstract
Collection protocol obtained from the attached BioNet Specimen Procurement Agreement provided by the UMN CTSI Biorepository and Laboratory Services (BLS).
Tissue Procurment Agreement - 06.2023.pdf343KB Troubleshooting
Preparation
Patient Identification: As soon as a patient is scheduled, the research team will email [email protected] a completed Specimen Procurement Request Form.
Patient Consent: Researcher consents. The original signed consent form will be placed in the patient chart and scanned into Epic. BioNet will verify consent in Epic. If the consent is not scanned in EPIC before the procedure, the researcher will provide BioNet a copy direct (e-mail or hard copy).
Collection Supplies: All supplies will be delivered to the BioNet office at least one day before the procedure and appropriately labeled with researcher name and CTSI Project #.
Collection Kit Components:
PBS (Study team)
Media: DMEM + antibiotics (Study team)
Media tubes (x3 per collection) (Study team)
10% Formalin (Bionet)
FFPE cassette x 5 (Bionet)
Specimen container (Bionet)
OCT compound (Bionet)
OCT mold x 4 (Bionet)
OCT quick freeze box (Bionet)
Liquid nitrogen vapor (Bionet)
Cryovials x 3 (Bionet)
Collection kit containing 10 ml EDTA blood tubes x 2 (Bionet)
Procurement and Processing of Blood Samples
- Study team will set up blood processing instructions with TTL.
- Study team will place order for blood in EPIC.
- Study team will provide preop staff with blood tube collection kits. 2 x 10 ml EDTA blood tubes will be in a bag labeled with BLS pager number for pick up contact.
- Blood tubes will be held at room temperature until delivery to TTL or pick up by study team.
- BLS will pick up blood tubes and submit to TTL for processing. If filled blood tubes are returned to BLS past TTL’s 2pm processing deadline, study team will be notified to pick up blood tubes and process through their lab.