The aim with this protocol is to pull together what we have learnt from dissociating other tissues, to generate a 'generic' protocol that can be used on multiple tissues for single cell RNA sequencing. To date (June 2019) we have mainly used this on human adult lung and gut and it is definitely still in development! We are also testing replacing liberase TL with liberase DH. The trypsin step may or may not be necessary depending on tissue, but can liberate additional cells compared to the liberase alone.