Apr 29, 2020
HuBMAP UF TMC - 10x Genomics scRNAseq Modality Overview
- 1University of Florida
- Human BioMolecular Atlas Program (HuBMAP) Method Development CommunityTech. support email: Jeff.spraggins@vanderbilt.edu
Protocol Citation: Marda Jorgensen, Maigan Brusko 2020. HuBMAP UF TMC - 10x Genomics scRNAseq Modality Overview . protocols.io https://dx.doi.org/10.17504/protocols.io.be79jhr6
License: This is an open access protocol distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
Protocol status: Working
We use this protocol and it's working
Created: April 16, 2020
Last Modified: October 02, 2020
Protocol Integer ID: 35809
Safety warnings
Follow all safety guidelines provided with individual links within this protocol.
Specimen/Organ Prep:
Lymph Node: dx.doi.org/10.17504/protocols.io.bbgnijve
Single Cell Isolation/Storage: dx.doi.org/10.17504/protocols.io.bd9vi966
Optional (recommended) step: FACS isolation of live single cells for scRNASeq: dx.doi.org/10.17504/protocols.io.bfqzjmx6
10x Library Construction and Sequencing: dx.doi.org/10.17504/protocols.io.bffvjjn6
Data Analysis and Quality Control Check 
10x Cell Ranger.docx
10x Cell Ranger.docx Register completed 10x sample information in the HuBMAP UUID generator at https://uuid.hubmapconsortium.org.
Prepare and upload data to the HuBMAP HIVE Globus using hipergator.
HPG 10x (1).docx