Feb 28, 2022
HuBMAP | GE/URMC/SCH Cell DIVE™ Modality Overview
- Gloria Pryhuber1,
- gail.deutsch 2,
- Christine Surrette3,
- Lisa Lowery3,
- heidie_huyck 4,
- Cory Poole4
- 1University of Rochester Medical Center;
- 2Seattle Children's Hospital;
- 3GE Research;
- 4University of Rochester
- GE Research
Protocol Citation: Gloria Pryhuber, gail.deutsch , Christine Surrette, Lisa Lowery, heidie_huyck , Cory Poole 2022. HuBMAP | GE/URMC/SCH Cell DIVE™ Modality Overview. protocols.io https://dx.doi.org/10.17504/protocols.io.b4kgqutw
License: This is an open access protocol distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
Protocol status: Working
We use this protocol and it’s working
Created: February 02, 2022
Last Modified: February 28, 2022
Protocol Integer ID: 57704
Keywords: sch cell dive collaboration, human biomolecular atlas program, hubmap, links to each of the individual protocol, urmc, overview of all protocol
Abstract
This is an overview of all protocols currently in use for the GE/URMC/SCH Cell DIVE collaboration for the Human BioMolecular Atlas Program (HuBMAP). It includes links to each of the individual protocols that make up this project workflow.
Troubleshooting
Confirm donor acceptance criteria for inclusion.
Prepare paraffin blocks and FFPE sections from tissue samples.
Characterize antibodies (primary/secondary, direct conjugates, and zenon labelled antibodies) and determine any antigen effects from the Cell DIVE dye inactivation process.
Prepare direct conjugates for study.
Perform Cell DIVE™ multiplexed data acquisition on the final cohort.
Note
Staining is done using the Leica Bond MAX and images are acquired on the IN Cell Analyzer 2200.