License: This is an open access protocol distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
Protocol status: Working
We use this protocol and it's working
Created: March 21, 2024
Last Modified: March 22, 2024
Protocol Integer ID: 97070
Funders Acknowledgements:
HuBMAP
Grant ID: U54 AR081775-01
Disclaimer
The protocols.io team notes that research involving animals and humans must be conducted according to internationally-accepted standards and should always have prior approval from an Institutional Ethics Committee or Board.
Abstract
This is an overview of all protocols currently in use for the 2024 GE HealthCare/UPitt collaboration for the Human BioMolecular Atlas Program (HuBMAP). It includes links to each of the individual protocols that make up this project workflow.
5 mm skin biopsies obtained from donor subjects are divided into two parts, with one half for formalin fixed and paraffin embedded (FFPE) processing and the other half for skin digestion for further scRNA analysis.
Characterize antibodies (primary/secondary, direct conjugates, and zenon labelled antibodies) and determine any antigen effects from the Cell DIVE dye inactivation process.