GoTaq\u00ae G2 Green Master Mix(a,b)\u00a0is a premixed ready-to-use solution containing GoTaq\u00ae G2 DNA Polymerase, dNTPs, MgCl2 and reaction buffers at optimal concentrations for efficient amplification of a wide range of DNA templates by PCR. GoTaq\u00ae G2 Green Master Mix contains two dyes (blue and yellow) that allow monitoring of progress during electrophoresis. Reactions assembled with GoTaq\u00ae G2 Green Master Mix have sufficient density for direct loading onto agarose gels. GoTaq\u00ae G2 DNA Polymerase exhibits 5 \u21923 exonuclease activity. GoTaq\u00ae G2 Green Master Mix is recommended for any amplification reaction that will be visualized by agarose gel electrophoresis and ethidium bromide staining. The master mix is not recommended if any downstream applications use absorbance or fluorescence excitation, as the yellow and blue dyes in the reaction buffer may interfere with these applications. The dyes absorb wavelengths in the range of 225\u2013300nm, making standard A260 readings to determine DNA concentration unreliable. The dyes have excitation peaks at 488nm and in the range of 600\u2013700nm that correspond to the excitation wavelengths commonly used in fluorescence detection instrumentation. For some instrumentation, such as a fluorescent gel scanner that uses a 488nm excitation wavelength, there will be minimal interference since it is the yellow dye that absorbs at this wavelength. Gels scanned by this method will have a light grey dye front (corresponding to the yellow dye front) below the primers.