Rapid, cheap, and effective DNA extraction for PCR. Easily scaled up to 96-well plates. This originates with Truett et al (2000) PMID:10907076This protocol is very well tested by many labs for subsequent PCR amplification. For PCR it does not matter that DNA also has protein, is in low concentration, and is fragmented. This is a very robust DNA preparation method.It is not difficult to do 960 DNA extractions per day (10 plates), the major determinant is tissue handling. AB1 Alkaline Lysis Solution (pH 12)For 100 ml add 225 mM NaOH 25 ml of 100 mM NaOH 30.2 mM Na2EDTA 0.4 ml of 50 mM Na2EDTA (pH 8) 4 74.6 ml of ddH2O AB1 Neutralizing Solution (pH 5)For 100 ml add: 240 mM Tris-HCl 630 mg of Tris-HCl 3 100 ml of ddH2O Notes: Prepare alkaline lysis solution fresh each day Neutralizing solution is stable at room temperature for long periods (months-years) Tris-HCL is NOT Tris-base The main cause of failure is too much tissue for the volume of alkaline lysis solution