Apr 30, 2026

Homework2 - Rehydrating and resuspending primers in solution

DOI
https://dx.doi.org/10.17504/protocols.io.ewov1r6x2lr2/v1
  • Hieu Nguyen1,
  • Chenyi Jiang1
  • 1Boston University
Hieu Nguyen
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DOI: https://dx.doi.org/10.17504/protocols.io.ewov1r6x2lr2/v1
Protocol Citation: Hieu Nguyen, Chenyi Jiang 2026. Homework2 - Rehydrating and resuspending primers in solution. protocols.io https://dx.doi.org/10.17504/protocols.io.ewov1r6x2lr2/v1
License: This is an open access  protocol  distributed under the terms of the  Creative Commons Attribution License,  which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
Protocol status: Other
Have not been tested yet
Created: April 30, 2026
Last Modified: April 30, 2026
Protocol  Integer ID: 315993
Keywords: resuspending primer, primers in solution, primer in solution
Abstract
Preparing the frozen primer
30m
Get the dried primer
Record the nmol of the primer from the documentation

Place dried primer into vile/tube if it is not already in it
Place vile/tube with dried primer into the centrifuge
Balance the centrifuge
3000 rpm, 4°C, 00:15:00

30m
Stop the centrifuge and carefully take out the vile/tube without mixing up the primer
Making Stock Solution
For each nmol of primer, add 10 µL of water to make the stock solution

Creating Working Solution
Take 10 µL of stock concentration and mix it with 90 µL of buffer

Clean Up
Store away the Stock Solution for later use