Feb 06, 2023
HEPES transformation buffer
This protocol is a draft, published without a DOI.
- 1University of Oslo
Protocol Citation: Andreas Sagen 2023. HEPES transformation buffer. protocols.io https://dx.doi.org/
License: This is an open access protocol distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
Protocol status: Working
We use this protocol and it's working
Created: February 05, 2023
Last Modified: February 06, 2023
Protocol Integer ID: 76420
Keywords: transformation buffer, HEPES buffer, electroporation, mechanical transformation, hepes buffer, cells for electroporation, hepes transformation, buffer, cell
Abstract
HEPES buffer is commonly used to prepare prokaryotic (bacterial) cells for electroporation.
Materials
LAF bench
Scale
Filter unit
Protocol materials
HEPESMerck MilliporeSigma (Sigma-Aldrich)Catalog #H4034
1 M HEPES buffer
Add 40 mL distilled water to a 50 mL tube
Measure and add 11.915 g HEPES
Materials:
HEPESMerck MilliporeSigma (Sigma-Aldrich)Catalog #H4034
Adjust pH to 7.4 with concentrated sodium hydroxide or hydrogen chloride
Add distilled water to 50 mL
Filter sterilize with a 0.2 µm pore-size filter