Aug 10, 2020
Hemolysis measurement of plasma/serum samples with Nanodrop ND1000
- 1Center for Medical Genetics Ghent
- CMGG
Protocol Citation: Hetty Helsmoortel 2020. Hemolysis measurement of plasma/serum samples with Nanodrop ND1000. protocols.io https://dx.doi.org/10.17504/protocols.io.t3teqnn
Manuscript citation:
License: This is an open access protocol distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
Protocol status: Working
We use this protocol and it's working
Created: September 28, 2018
Last Modified: August 10, 2020
Protocol Integer ID: 16211
Keywords: hemolysis
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Abstract
This protocol describes how to measure hemolysis on plasma or serum samples.
Before you start
Before you start
Place: room 120.042, 2nd floor MRB1
- Aliquot 3 ul plasma sample in a separate tube (can be left at room temperature)
- Open sample arm and clean sample pedestal with an ethanol-dipped swab (dust-free). Swabs are standing next to the machine.
- Clean the sample pedestal with a dry swab (dust-free).
Preparing hemolysis measurement
Preparing hemolysis measurement
- start ND-1000 software
- choose ‘Cell cultures’
- change max. absorbance from 0.2 to 2 (rescales the vertical axis)
- change wavelength (lambda) to 414 nm
Load blank sample
Load blank sample
- pipet 1 ul water to the sample pedestal, using reverse pipetting to avoid bubbles (use an unused water tube)
- gently close the sample arm
- click ‘blank’ to calibrate the machine. If the wave shows peaks, repeat the pipetting and re-blank
Measure blank sample
Measure blank sample
- open sample arm and clean pedestal with a dust-free swab
- (to have a good reference, the first sample we measure is always a water sample)
- pipet 1 ul water to the sample pedestal, using reverse pipetting to avoid bubbles (use an unused water tube)
- gently close the sample arm
- click ‘measure’ to measure water sample
Measure plasma/serum sample(s)
Measure plasma/serum sample(s)
- open sample arm and clean pedestal with a dust-free swab
- pipet 1 ul plasma sample to the sample pedestal, using reverse pipetting to avoid bubbles
- gently close the sample arm
- click ‘measure’ to measure first sample
- (repeat steps above for other samples)
- clean sample pedestal and close sample arm
Export results
Export results
- choose ‘Reports’ > Export Report Table Only and save to USB stick (only Excel table, no full report with wavelength plots)