Jul 23, 2020
Hematoxylin and Eosin Staining Protocol
This protocol is a draft, published without a DOI.
- 1University of California, San Diego
- George Lab @ UCSDTech. support email: olgeorge@ucsd.edu
Protocol Citation: Sierra Simpson, Olivier George 2020. Hematoxylin and Eosin Staining Protocol. protocols.io https://protocols.io/view/hematoxylin-and-eosin-staining-protocol-bbyiipue
License: This is an open access protocol distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
Protocol status: Working
We use this protocol and it's working
Created: January 29, 2020
Last Modified: July 23, 2020
Protocol Integer ID: 32490
Materials
STEP MATERIALS
1L Mayer's Hematoxylin Solution, ModifiedG-BiosciencesCatalog #786-1264
Shandon™ Eosin Y Cytoplasmic Counterstain, AlcoholicThermo FisherCatalog #6766007
Protocol materials
1L Mayer's Hematoxylin Solution, ModifiedG-BiosciencesCatalog #786-1264
Shandon™ Eosin Y Cytoplasmic Counterstain, AlcoholicThermo FisherCatalog #6766007
1L Mayer's Hematoxylin Solution, ModifiedG-BiosciencesCatalog #786-1264
Shandon™ Eosin Y Cytoplasmic Counterstain, AlcoholicThermo FisherCatalog #6766007
Prepare 95% ETOH in slide staining apparatus
Heomtoxylin in slide staining apparatus
Eosin in slide staining apparatus
Stain in Mayer's Hematoxylin
1L Mayer's Hematoxylin Solution, ModifiedG-BiosciencesCatalog #786-1264
Rinse slides 3X 15 min in tap water - if tap water is acidic, prepare Scott's tap water recipe in sigma insert
Rinse with DI water 30 Seconds
Place slide in 95% Ethanol for 1min
Place in Eosin Y for 1 min
Shandon™ Eosin Y Cytoplasmic Counterstain, AlcoholicThermo FisherCatalog #6766007
Dehydrate and clear through 2 changes each
95% Ethanol 2 minutes
100% Ethanol 2 minutes
Xylene ( Histoclear as a substitute ) EMS Cat# 64114
Mount with resinous mounting medium 200ul / slide.