Jul 07, 2020
HBSS/HEPES for ROS measurement in PMN
- Marco Cosentino1,
- Elisa Storelli1,
- Alessandra Luini1,
- Massimiliano LM Legnaro1,
- Emanuela Rasini1,
- Marco Ferrari1,
- Franca Marino1
- 1Center for Research in Medical Pharmacology, University of Insubria (Varese, Italy)
Document Citation: Marco Cosentino, Elisa Storelli, Alessandra Luini, Massimiliano LM Legnaro, Emanuela Rasini, Marco Ferrari, Franca Marino 2020. HBSS/HEPES for ROS measurement in PMN. protocols.io https://dx.doi.org/10.17504/protocols.io.bhdcj22w
License: This is an open access document distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
Created: June 10, 2020
Last Modified: July 07, 2020
Document Integer ID: 38020
HBSS/HEPES x ROS composition (g/l):
NaCl: 4.2300 g
KCl: 0.1864 g
MgSO4: 0.1232 g
CaCl2: 0.735 g
Glucose: 0.900 g
Hepes: 1.300 g
To prepare 0.5 L of HBSS/HEPES, dissolve the reagents listed above in 450 mL of ultrapure H2O.
Adjust the pH to 7.4 with HCl or NaOH, and then add H2O to 0.5 L.
Storage: 4°C Fridge 1- (Room TSO8)
NaCl code: S9625, Sigma
KCl code: P9541, Sigma
MgSO4 code: 1.05886, Sigma
CaCl2 code: 1.02382 Sigma
Glucose code: 1.08337, Sigma
Hepes code: H7006 Sigma