Dec 01, 2023
Habituation and dishabituation (olfaction test)
- 1Vall d'Hebron Research Institute
- Vilalab Public
- Nuria
External link: https://doi.org/10.1038/s41467-024-53168-7
Protocol Citation: Marina Lorente Picón, Núria Peñuelas, Ariadna Laguna, Miquel Vila 2023. Habituation and dishabituation (olfaction test). protocols.io https://dx.doi.org/10.17504/protocols.io.e6nvwdq9dlmk/v1
Manuscript citation:
Laguna, A., Peñuelas, N., Gonzalez-Sepulveda, M. et al. Modelling human neuronal catecholaminergic pigmentation in rodents recapitulates age-related neurodegenerative deficits. Nat Commun 15, 8819 (2024). https://doi.org/10.1038/s41467-024-53168-7
License: This is an open access protocol distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
Protocol status: Working
We use this protocol and it's working
Created: November 30, 2023
Last Modified: December 01, 2023
Protocol Integer ID: 91638
Keywords: habituation, olfaction test, mice, dishabituation, test
Abstract
Habituation and dishabituation (olfaction test) for mice
Individualize each mouse in a different cage with new bedding.
Present one cotton swab to the animal for object habituation for 10 min.
Present a second swab impregnated with water for 3 min.
Measure the number of times the animal goes towards the second cotton swab and the time the animal spends sniffing it.
Present a third swab impregnated with lemon essence (Essenciales) for 3 min.
Measure the number of times the animal goes towards the third cotton swab and the time the animal spends sniffing it.
Calculate the discrimination index (DI) according to the formula: (Time exploring lemon essence – Time exploring water) / (Time exploring lemon essence + Time exploring water).