Invitrogen's HA tag monoclonal antibody in combination with Western Breeze Anti-Mouse Chemiluminescent Western Blot Immunodetection Kit leads to no background Western blots with Pt lysates. HA tag thus represents a tag of choice for (1) efficient and unambioguous protein expression analysis, (2) immunofluorescence experiments and (3) various IP and pull-down experiments in Pt and possibly other diatoms as well as other marine microeukaryotes.