Media for V. natriegens LB2: Standard LB media (10.0 g/L Tryptone, 5.0 g/L Yeast Extract, 10.0 g/L NaCl) with additional 1.5% NaCl LBv2 Salts: Standard LB media (10.0 g/L Tryptone, 5.0 g/L Yeast Extract, 10.0 g/L NaCl) with additional with additional v2 salts (204 mM NaCl, 4.2 mM KCl, and 23.14 mMMgCl2) BHIv2: Brain heart infusion Broth (37 g/L Teknova Brain Heart Infusion Broth Dry Media (Cat. No. B9500) with additional v2 salts (204 mM NaCl, 4.2 mM KCl, and 23.14 mM MgCl2) note: BHI can not be autoclaved together with the salts, they have to be autoclave separate and later on added. Matthew T Weinstock, Eric D Hesek, Christopher M Wilson, Daniel G GibsonVibrio natriegens as a fast-growing host for molecular biology Nature Methods volume 13, pages 849–851 (2016)