May 05, 2026
Growing Bacteria From NaOH Crystals
- Dominika Papaj1
- 1Western University
Protocol Citation: Dominika Papaj 2026. Growing Bacteria From NaOH Crystals. protocols.io https://dx.doi.org/10.17504/protocols.io.bp2l6jq11vqe/v1
License: This is an open access protocol distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
Protocol status: Working
We use this protocol and it's working
Created: May 04, 2026
Last Modified: May 05, 2026
Protocol Integer ID: 316324
Keywords: bacteria from naoh crystal, growing bacteria, naoh crystal, bacteria
Abstract
This is a protocol to explain how bacteria growing on NaOH crystals were grown and isolated.
Growing Bacteria From NaOH Crystals
A 0.5g sample of the sodium hydroxide crystals was suspended in 2mL of deionized water in a glass vial
Crystals were crushed in the vial to release any bacteria that may have been stuck in the crystals
DI water and sodium hydroxide crystals were mixed by hand for 5 minutes
Using a micropipette a 900 µL sample of the water and crystals was pipetted on the surface of LB agar at pH 7
Using the spread plate technique and a sterile spreader the liquid was spread around the plate
The medium was incubated aerobically at 35°C for 4 days to produce colonies