Nov 23, 2023
- Marina Lorente Picón1,
- Núria Peñuelas1,
- Ariadna Laguna1,
- Miquel Vila1
- 1Vall d'Hebron Research Institute
- Vilalab Public
- Nuria
External link: https://doi.org/10.1038/s41467-024-53168-7
Protocol Citation: Marina Lorente Picón, Núria Peñuelas, Ariadna Laguna, Miquel Vila 2023. Grip strength test. protocols.io https://dx.doi.org/10.17504/protocols.io.261gedjjwv47/v1
Manuscript citation:
Laguna, A., Peñuelas, N., Gonzalez-Sepulveda, M. et al. Modelling human neuronal catecholaminergic pigmentation in rodents recapitulates age-related neurodegenerative deficits. Nat Commun 15, 8819 (2024). https://doi.org/10.1038/s41467-024-53168-7
License: This is an open access protocol distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
Protocol status: Working
We use this protocol and it's working
Created: November 23, 2023
Last Modified: November 23, 2023
Protocol Integer ID: 91354
Keywords: grip strength test grip strength test, mice, strength, test
Abstract
Grip strength test for mice
Troubleshooting
Hold the animal by the middle/base of the tail and
allow it to grasp a tangled fine gauge stainless steel wire attached to steel
chain (13.2g). Then, lift them carrying the corresponding weight with
their forepaws for a total of 5 seconds. If the animal does not succeed assign 0
seconds to that animal. If the animal succeeds holding the weight, move on to the next weight.
Hold the animal by the middle/base of the tail and
allow it to grasp a tangled fine gauge stainless steel wire attached to steel
chain (32.1g). Then lift them carrying the corresponding weight with
their forepaws for a total of 5 seconds. If the animal does not succeed assign the max nº of seconds he held the weight to that
animal. If the animal succeeds holding the weight, move on to the next weight.
Hold the animal by the middle/base of the tail and
allow it to grasp a tangled fine gauge stainless steel wire attached to steel
chain (19.7g). Then lift them carrying the corresponding weight with
their forepaws. If the animal does not succeed assign the max nº of secondsto that animal. If the animal succeeds holding the weight, move on to the next weight.
Hold the animal by the middle/base of the tail and
allow it to grasp a tangled fine gauge stainless steel wire attached to steel
chain (25.9g). Then lift them carrying the corresponding weight with
their forepaws. If the animal does not succeed assign the max nº of secondsto that animal. If the animal succeeds holding the weight, move on to the next weight.
Hold the animal by the middle/base of the tail and
allow it to grasp a tangled fine gauge stainless steel wire attached to steel
chain (38.4g). Then lift them carrying the corresponding weight with
their forepaws. If the animal does not succeed, assign the max nº of secondsto that animal. If they succeed holding the weight, move on to the next weight.
Hold the animal by the middle/base of the tail and
allow it to grasp a tangled fine gauge stainless steel wire attached to steel
chain (44.6g). Then lift them carrying the corresponding weight with
their forepaws. If the animal does not succeed, assign the max nº of secondsto that animal. If the animal succeeds holding the weight, assign 30 seconds to that animal.
Calculate Grip latency (s) as a sum of the time holding the increasing weights (0-30 seconds).