gmcSCRB-seq is an alternative lysis protocol to mcSCRB-seq (Publication and Protocol). gmcSCRB-seq uses Guanidine Hydrochloride in the lysis buffer and requires an additional cean up step. We recommend using this alternative lysis protocol in cases where cells are difficult to lyse or where the DNA appears degraded following the pre-amplification step. However, we have observed reduced sensitivity with gmcSCRB-seq compared to mcSCRB-seq and would therefore only recommend using it in cases where the latter does not produce high quality libraries.