Apr 18, 2024
Version 2
Glucose Tolerance Test V.2
- Sabina Marciano1,2,
- Roberta Marongiu1,2
- 1Department of Neurological Surgery, Weill Cornell Medical College, New York, NY 10065;
- 2Aligning Science Across Parkinson’s (ASAP) Collaborative Research Network, Chevy Chase, MD 20815, USA
Protocol Citation: Sabina Marciano, Roberta Marongiu 2024. Glucose Tolerance Test. protocols.io https://dx.doi.org/10.17504/protocols.io.261geddodv47/v2Version created by Eileen Ruth Torres
License: This is an open access protocol distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
Protocol status: Working
We use this protocol and it's working
Created: April 18, 2024
Last Modified: September 23, 2024
Protocol Integer ID: 98408
Keywords: ASAPCRN
Funders Acknowledgements:
Aligning Science Across Parkinson's
Grant ID: 020608
Abstract
The glucose tolerance test is performed to determine how quickly the glucose is cleared from the blood. It is used to test for diabetes or insulin resistance.
Single cage the mice for 1 week in advance
Weight the mice
Fast mice for 06:00:00
6h
After fasting, measure glucose with a glucose meter
Perform an intraperitoneal injection of 2g / kg body weight of glucose (20% D-glucose stock solution dissolving 2g of glucose in 10ml saline and give 10ul per gram of body weight).
Collect blood samples for glucose measurement at 15, 30, 45, 60, 90 and 120 min post glucose injection