Apr 03, 2023

Public workspaceGlucocerebrosidase is imported into mitochondria and preserves complex I integrity and energy metabolism - ASAP protocol collection

  • michela.deleidi 1,
  • Pascale Baden1,
  • Maria Jose Perez J.1,
  • Hariam Raji1,
  • Federico Bertoli1
  • 1German Center for Neurodegenerative Diseases (DZNE), Tübingen, 72076 Germany
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Collection Citationmichela.deleidi , Pascale Baden, Maria Jose Perez J., Hariam Raji, Federico Bertoli 2023. Glucocerebrosidase is imported into mitochondria and preserves complex I integrity and energy metabolism - ASAP protocol collection. protocols.io https://dx.doi.org/10.17504/protocols.io.x54v9d44mg3e/v1
License: This is an open access collection distributed under the terms of the Creative Commons Attribution License,  which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
Protocol status: Working
We use this collection and it's working
Created: April 03, 2023
Last Modified: May 31, 2024
Collection Integer ID: 79922
Keywords: ASAPCRN, Glucocerebrosidase is imported into mitochondria and preserves complex I integrity and energy metabolism, glucocerebrosidase, mitochondria, energy metabolism, deleidi lab
Funders Acknowledgements:
Aligning Science Across Parkinson’s through the Michael J. Fox Foundation for Parkinson’s Research (MJFF)
Grant ID: ASAP-000420
EU Joint Program—Neurodegenerative Disease Research (JPND) project
Grant ID: GBA-PaCTS, 01ED2005B
Abstract
Collection of protocols of Deleidi Lab used in the publication: "Glucocerebrosidase is imported into mitochondria and preserves complex I integrity and energy metabolism"
Troubleshooting
Files
Protocol
Name
Gcase co-immunoprecipitation
Version 1
, ASAP
Federico BertoliASAP
Protocol
Name
Single cell dissociation of brain organoids
Version 1
, ASAP
Federico BertoliASAP
Protocol
Name
Plasmid-reprogramming of human fibroblasts
Version 1
, ASAP
Federico BertoliASAP
Protocol
Name
Sequencing of construct
Version 1
, ASAP
Federico BertoliASAP
Protocol
Name
Single cell analysis of iPSC-derived midbrain organoids
Version 1
, ASAP
Federico BertoliASAP
Protocol
Name
Complex I activity assay
Version 1
, ASAP
Federico BertoliASAP
Protocol
Name
Midbrain-like Organoids generation from hiPSCs
Version 1
, ASAP
Federico BertoliASAP
Protocol
Name
Endogenous coimmunoprecipitation
Version 1
, ASAP
Federico BertoliASAP
Protocol
Name
Generation of induced pluripotent stem cells and gene correction
Version 1
, ASAP
Federico BertoliASAP
Protocol
Name
Virus production
Version 1
, ASAP
Federico BertoliASAP
Protocol
Name
Live-cell imaging
Version 1
, Institut Imagine Paris - Inserm U1163
Hariam RajiInstitut Imagine Paris - Inserm U1163
Protocol
Name
Expansion microscopy
Version 1
, ASAP
Federico BertoliASAP
Protocol
Name
Western Blot Analysis
Version 1
, ASAP
Federico BertoliASAP
Protocol
Name
Soluble and insoluble A-SYN fractionation
Version 1
, ASAP
Federico BertoliASAP
Protocol
Name
Mitochondrial complex activity assays
Version 1
, ASAP
Federico BertoliASAP
Protocol
Name
Neuromelanin staining (Fontana-Masson staining)+ TH-DAB staining on midbrain organoids
Version 1
, ASAP
Federico BertoliASAP
Protocol
Icon representing the file Differentiation	NPCs	to Dopaminergic/Midbrain Neurons
Name
Differentiation NPCs to Dopaminergic/Midbrain Neurons
Version 1
, ASAP
Federico BertoliASAP
Protocol
Name
Immunofluorescence staining
Version 1
, Institut Imagine Paris - Inserm U1163
Hariam RajiInstitut Imagine Paris - Inserm U1163
Protocol
Name
In vitro GCase activity assay (total cell lysate)
Version 1
, ASAP
Federico BertoliASAP
Protocol
Name
Flag co-immunoprecipitation
Version 1
, ASAP
Federico BertoliASAP
Protocol
Name
Constructs and generation of stable cell lines
Version 1
, ASAP
Federico BertoliASAP