Oct 12, 2023

Public workspaceGI Transit Assay

DOI
https://dx.doi.org/10.17504/protocols.io.eq2lyjj3mlx9/v1
  • Roberta Marongiu1,2,
  • Sabina Marciano3
  • 1Department of Neurological Surgery, Weill Cornell Medical College, New York, NY 10065;
  • 2Aligning Science Across Parkinson’s (ASAP) Collaborative Research Network, Chevy Chase, MD 20815, USA;
  • 3Weill Cornell medicine
Jacquelyn Haytayan
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DOI: https://dx.doi.org/10.17504/protocols.io.eq2lyjj3mlx9/v1
Protocol Citation: Roberta Marongiu, Sabina Marciano 2023. GI Transit Assay. protocols.io https://dx.doi.org/10.17504/protocols.io.eq2lyjj3mlx9/v1
License: This is an open access protocol distributed under the terms of the Creative Commons Attribution License,  which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
Protocol status: Working
We use this protocol and it's working
Created: August 14, 2023
Last Modified: September 23, 2024
Protocol Integer ID: 86471
Keywords: ASAPCRN, gi transit assay this test, gi transit assay, alteration in the gut transit, gut transit, constipation, possible signs of constipation, signs of constipation, test, possible sign
Abstract
This test is used to check for possible signs of constipation and alteration in the gut transit
Materials
Carmine red (Sigma-Aldrich, St. Louis MO)

Troubleshooting
Fast mice for 6 hours
Administer 0.3 mL Carmine red per mouse via oral gavage
Monitor the mice every 15 minutes for up to 5 hours for the appearance of the first red fecal pellet
The time from gavage to passage of first red pellet is recorded as total intestinal transit time.