Collection Citation: Christian CR Renicke, Natalie Swinhoe, Catherine Henderson, Emily K. Meier, Phillip Cleves, Lorraine Ling, Sophia Gaupp . Genetic tools for the sea anemone Aiptasia. protocols.io https://dx.doi.org/10.17504/protocols.io.q26g7nzbqlwz/v1
Manuscript citation:
Renicke C, Swinhoe N, Henderson C, Meier E, Ling L, Keat GL, Maruyama S, Rangarajan-Paul M, Pringle JR, Cleves PA. 2025. Development of genetic tools for the sea anemone Aiptasia, a model system for coral biology. Goldstein B, editor. GENETICS. 231(3):iyaf194. doi:10.1093/genetics/iyaf194.
License: This is an open access collection distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
Created: June 28, 2025
Last Modified: September 04, 2025
Collection Integer ID: 221255
Keywords: genetic tools for the sea anemone aiptasia, sea anemone aiptasia, small sea anemone aiptasia, genetic tool, use of genetic tool, exaiptasia diaphana, aiptasia, genetic construct, larvae, general aiptasia husbandry, plasmid dna, guidelines for general aiptasia husbandry, electroporation into zygote, dna, spawning set, shrna
Funders Acknowledgements:
Simons Foundation
Grant ID: LIFE677354
National Science Foundation
Grant ID: NSF-IOS EDGE #1645164
Stanford School of Medicine
Grant ID: Discovery and Innovation fund
National Science Foundation
Grant ID: NSF-IOS EDGE #2128073
Carnegie Institution for Science
Grant ID: Start-up funds
Moore Foundation
Revive and Restore
Pew
Grant ID: Biomedical and Marine Fellow Award
Disclaimer
DISCLAIMER – FOR INFORMATIONAL PURPOSES ONLY; USE AT YOUR OWN RISK
The protocol content here is for informational purposes only and does not constitute legal, medical, clinical, or safety advice, or otherwise; content added to protocols.io is not peer reviewed and may not have undergone a formal approval of any kind. Information presented in this protocol should not substitute for independent professional judgment, advice, diagnosis, or treatment. Any action you take or refrain from taking using or relying upon the information presented here is strictly at your own risk. You agree that neither the Company nor any of the authors, contributors, administrators, or anyone else associated with protocols.io, can be held responsible for your use of the information contained in or linked to this protocol or any of our Sites/Apps and Services.
Abstract
This is a collection of protocols for use of genetic tools in the small sea anemone Aiptasia (Exaiptasia diaphana). The protocols give step-by-step guides for production of genetic constructs (plasmid DNA, capped mRNA and shRNA), electroporation into zygotes and techniques for subsequent analysis of the larvae. They also include updated guidelines for general Aiptasia husbandry and spawning set-ups.
Troubleshooting
DISCLAIMER – FOR INFORMATIONAL PURPOSES ONLY; USE AT YOUR OWN RISK
The protocol content here is for informational purposes only and does not constitute legal, medical, clinical, or safety advice, or otherwise; content added to protocols.io is not peer reviewed and may not have undergone a formal approval of any kind. Information presented in this protocol should not substitute for independent professional judgment, advice, diagnosis, or treatment. Any action you take or refrain from taking using or relying upon the information presented here is strictly at your own risk. You agree that neither the Company nor any of the authors, contributors, administrators, or anyone else associated with protocols.io, can be held responsible for your use of the information contained in or linked to this protocol or any of our Sites/Apps and Services.
