Generation of Tn5 transposome, protein purification and loading for the sci- family of protocols.Protein purification of pTXB1.Tn5 transformed into T7 Express LysY (NEB C3031) cells; reference is PTXB1-Tn5 Addgene #60240 from Picelli et al. Genome Res. 2014.''This protocol is built largely off of work described inPicelli, Simone, et al. "Tn5 transposase and tagmentation procedures for massively scaled sequencing projects." Genome research 24.12 (2014): 2033-2040.