Universal 16S rRNA probe-based-qPCR assay for bacteria.The primers target the V4 region of the 16S rRNA gene and were specifically designed for Illumina amplicon sequencing. The original primers were designed by Caporaso et al. (2012) and modified by Walters et al. (2015). For barcoding, we use the Fludigm Access Array for barcoding the sample and therefore the primers are synthisized with the CS1 and CS2 regions.