Oct 09, 2024

Public workspaceGEARBOCS protocols V.3

DOI
https://dx.doi.org/10.17504/protocols.io.36wgqnq15gk5/v3
  • Justin T Savage1,
  • Luke Bradley1,
  • Nicholas Brose1
  • 1Duke University
  • Eroglu_Lab
Justin T Savage
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DOI: https://dx.doi.org/10.17504/protocols.io.36wgqnq15gk5/v3
Collection Citation: Justin T Savage, Luke Bradley, Nicholas Brose 2024. GEARBOCS protocols. protocols.io https://dx.doi.org/10.17504/protocols.io.36wgqnq15gk5/v3Version created by Justin T Savage
License: This is an open access collection distributed under the terms of the Creative Commons Attribution License,  which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
Protocol status: Working
We use this collection and it's working
Created: October 09, 2024
Last Modified: October 09, 2024
Collection Integer ID: 109513
Keywords: ASAPCRN, gearbocs protocols protocols for aav production, gearbocs protocols protocol, use of the gearbocs system, gearbocs system, aav production, aav, protocol
Funders Acknowledgements:
Aligning Science Across Parkinson’s
Grant ID: ASAP-020607
Abstract
Protocols for AAV production and use of the GEARBOCS system.
Troubleshooting
Files
Protocol
Name
AAV Injection and Tissue Preparation
Version 2
, Duke University
Luke BradleyDuke University
Protocol
Name
ForkDesigning sgRNA Oligos and Inserting Guides into the GEARBOCS Vector
Version 1
, Duke University
Luke BradleyDuke University
Protocol
Name
Culturing 3T3 Cells for Validating GEARBOCS Constructs
Version 2
, Duke University
Luke BradleyDuke University
Protocol
Name
AAV Production and Purification
Version 3
, Duke University
Luke BradleyDuke University