May 09, 2025
Fusarium falciforme isolation from soil
This protocol is a draft, published without a DOI.
- 1Boyce Thompson Institute;
- 2Cornell University
Protocol Citation: Isako Di Tomassi 2025. Fusarium falciforme isolation from soil. protocols.io https://protocols.io/view/fusarium-falciforme-isolation-from-soil-gy5hbxy37
License: This is an open access protocol distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
Protocol status: Working
We use this protocol and it's working
Created: May 09, 2025
Last Modified: May 09, 2025
Protocol Integer ID: 217993
Abstract
Basic steps for isolating Fusarium falciforme from soil.
Dilute or sprinkle soil on to Nash-Snyder or Komada’s media, Van Wyck's agar is another that may work
After 48-72 hrs, ‘F. solani’ (and other fusaria) emerge
Look for long, straight monophialides with false heads, often arranged in a verticillate pattern at ~45 degree angles. What you find is likely to be F. solani sensu stricto and F. falciforme, and a few other possible species
Fig. 2. Morphological and microscopic characteristics of F. faclciforme
isolate, SP-Ff-Kd. (A and B) Mycelial growth and pigmentation on PDA
medium. (C) Hyaline and septate hypha. (D) Sporodochium. (E) Branched
phialides. (F) Unbranched phialides. (G) Septate macroconidia. (H) Long
conidiophore bearing branched and unbranched phialides with mass of
microconidia. (I) Septate and non-septate microconidia. (J)
Chlamydospores produced on PDA medium. (K) Hyaline, round and globose to
sub globose chlamydospores. (L–N) Terminal chlamydospores produced
singly, pair and in chain. (O–Q) Intercalary chlamydospores produced
singly, pair and in chain. (Madhavan)
Use Tef1a for distinguishing using the new Fusarium-ID
Contact Fusarium-ID makers if assistance is required for identifying F. falciforme
Protocol references
Personal correspondence with D. Geiser, October 25, 2023
Komada,H., 19771936599, English, Journal article, 8, Review of Plant Protection Research, (114–124), Development of a selective medium for quantitative isolation of Fusarium oxysporum from natural soil., (1975)
Nash,Shirley M. and Snyder,W. C., 19631100297, Undetermined, Journal article, 1943-7684 0031-949X, 52, (6), Phytopathology, (567–572 pp.), Quantitative estimations by plate counts of propagules of the Bean root rot Fusarium in field soils., (1962)
(2019). PM 7/91 (2) Fusarium circinatum (formerly Gibberella circinata). EPPO Bulletin, 49: 228–247. https://doi.org/10.1111/epp.12587
Madhavan, S., et al. "Characterization of Fusarium falciforme inciting wilt in peace lily (Spathiphyllum wallisii)." Scientia Horticulturae 339 (2025): 113834.
Bulitz, E. G. "A selective medium for the isolation of Fusarium spp. from soil debris." Phytophylactica 18.2 (1986): 67-70.
Torres-Cruz, Terry J., et al. "FUSARIUM-ID v. 3.0: An updated, downloadable resource for Fusarium species identification." Plant disease 106.6 (2022): 1610-1616.