Nov 20, 2020

Public workspaceFungal Plate Photography

DOI
dx.doi.org/10.17504/protocols.io.bnu6meze
  • Craig Bateman1
  • 1University of Florida
  • Protocols Bark Beetle Mycobiome
Bark Beetle Mycobiome Research Coordination Network Bark
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DOI: dx.doi.org/10.17504/protocols.io.bnu6meze
Document Citation: Craig Bateman 2020. Fungal Plate Photography. protocols.io https://dx.doi.org/10.17504/protocols.io.bnu6meze
License: This is an open access document distributed under the terms of the Creative Commons Attribution License,  which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
Created: October 23, 2020
Last Modified: November 20, 2020
Document Integer ID: 43646
Abstract
This protocol describes how to photograph fungal plates.

This protocol is part of the Bark Beetle Mycobiome (BBM) Research Coordination Network. For more information on the BBM international network: Hulcr J, Barnes I, De Beer ZW, Duong TA, Gazis R, Johnson AJ, Jusino MA, Kasson MT, Li Y, Lynch S, Mayers C, Musvuugwa T, Roets F, Seltmann KC, Six D, Vanderpool D, & Villari C. 2020. Bark beetle mycobiome: collaboratively defined research priorities on a widespread insect-fungus symbiosis. Symbiosis 81: 101–113 https://doi.org/10.1007/s13199-020-00686-9.
  1. Download Canon EOS Utility to your computer: https://www.usa.canon.com/internet/portal/us/home/support/self-help-center/eos-utility (Listed as “EOS Digital Solution Disk Software”)
  2. Ensure photo setup is as pictured (in the flow hood in Adam’s office on the 2nd floor):

  1. Hook your computer up to the camera to enable live shooting and direct photo downloads.
  2. Turn on the hood blower and wipe down all surfaces with 70% ethanol.
  3. Set the camera dial to M (Manual) and adjust to the following settings: Manual focus Large resolution (use Raw for poster photos) Shutter 1/100 F9.0 ISO 400 Spot metering Flash white balance setting WB Shift: B5 G2 As it appears on the EOS Utility:

  1. Set a plate open-side-up underneath the camera and manually focus the camera to the top of the agar.
  2. Add a piece of paper displaying the underlying plate number in the photo.
  3. Proceed taking photographs of the top and bottom of each plate. Settings do not need to be altered between plates/photographs.
  4. Save photographs to our lab drive (Forest Entomology:\Plate photos) and name them according to plate number (eg: “plate number XXXX.jpg”)
  5. Keep the plate at 4-10° C until ready for discard. Plates should be discarded into an autoclave bag. **Note on batteries for flash: Only use Panasonic eneloop batteries. All batteries should be equally charged. Disconnect batteries after use. Battery charger is in camera drawer in Hulcr lab.