Jul 23, 2025

FUNDIS DNA Extraction, PCR, Oxford Nanopore Technologies MinION Sequencing and data analysis.  V.2

DOI
https://dx.doi.org/10.17504/protocols.io.36wgqnweygk5/v2
  • 1FUNDIS, Dikarya LLC, NAMA, California State University, East Bay;
  • 2Biodiverse
Harte Singer
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DOI: https://dx.doi.org/10.17504/protocols.io.36wgqnweygk5/v2
Collection Citation: Harte Singer, Stephen Douglas Russell 2025. FUNDIS DNA Extraction, PCR, Oxford Nanopore Technologies MinION Sequencing and data analysis. . protocols.io https://dx.doi.org/10.17504/protocols.io.36wgqnweygk5/v2Version created by Harte Singer
License: This is an open access  collection  distributed under the terms of the  Creative Commons Attribution License,  which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
Protocol status: Working
We use this collection and it's working
Created: October 19, 2024
Last Modified: July 23, 2025
Collection  Integer ID: 110349
Keywords: DNA Barcoding, Fungi, Mycology, Nanopore, nrITS, Oxford Nanopore Technologies, MinION, PCR, oxford nanopore technologies minion sequencing, generating dna barcode sequences from fungal sample, fundis dna extraction, oxford nanopore technologies minion platform, dna extraction, fungal sample, oxford nanopore technology, generating dna barcode sequence, fungal, minion
Abstract
Generating DNA barcode sequences from fungal samples using the Oxford Nanopore Technologies MinION platform. Some methods adapted from Stephen D. Russell's protocols dx.doi.org/10.17504/protocols.io.36wgq7qykvk5/v3
Files
Protocol
Icon representing the file FUNDIS ONT Post-PCR Pooling & Purification for Fungal Barcoding
Name
FUNDIS ONT Post-PCR Pooling & Purification for Fungal Barcoding
Version 1
Created by
Image of Harte Singer, FUNDIS
Harte SingerFUNDIS
Protocol
Name
FUNDIS DNA Extraction
Version 2
Created by
Image of Harte Singer, FUNDIS
Harte SingerFUNDIS
Protocol
Name
FUNDIS Setting up PCR for rDNA ITS Barcoding of Fungi using ONT Nanopore
Version 2
Created by
Image of Harte Singer, FUNDIS
Harte SingerFUNDIS
Protocol
Name
DNAMA VSVC Guidebook
Version 3
Created by
Image of Harte Singer, FUNDIS
Harte SingerFUNDIS
Protocol
Icon representing the file FUNDIS ONT V14 Nanopore Adapter Ligation for Fungal DNA Barcoding Flongle 10.4.1
Name
FUNDIS ONT V14 Nanopore Adapter Ligation for Fungal DNA Barcoding Flongle 10.4.1
Version 1
Created by
Image of Harte Singer, FUNDIS
Harte SingerFUNDIS
Protocol
Icon representing the file FUNDIS version ONT dA-tailing for Fungal Barcoding
Name
FUNDIS version ONT dA-tailing for Fungal Barcoding
Version 1
Created by
Image of Harte Singer, FUNDIS
Harte SingerFUNDIS
Protocol
Name
FUNDIS Fungal Tissue Sampling for PCR
Version 2
Created by
Image of Harte Singer, FUNDIS
Harte SingerFUNDIS