Aug 09, 2023

Public workspaceFresh Frozen / OCT Tissue RNA Quality Evaluation -- University of Minnesota TMCs (ThermoFisher Scientific, Invitrogen - Purelink)

DOI
dx.doi.org/10.17504/protocols.io.dm6gpjpz8gzp/v1
  • ThermoFisher Scientific1,
  • Laura J Niedernhofer2,
  • David A Bernlohr2
  • 1https://www.thermofisher.com/us/en/home/technical-resources/contact-us.html?enableChat=true;
  • 2University of Minnesota, Minneapolis, MN USA
  • Cellular Senescence Network (SenNet) Method Development Community
Mickayla DuFresne-To
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DOI: dx.doi.org/10.17504/protocols.io.dm6gpjpz8gzp/v1
Protocol Citation: ThermoFisher Scientific, Laura J Niedernhofer, David A Bernlohr 2023. Fresh Frozen / OCT Tissue RNA Quality Evaluation -- University of Minnesota TMCs (ThermoFisher Scientific, Invitrogen - Purelink). protocols.io https://dx.doi.org/10.17504/protocols.io.dm6gpjpz8gzp/v1
License: This is an open access protocol distributed under the terms of the Creative Commons Attribution License,  which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
Protocol status: Working
We use this protocol and it's working
Created: May 03, 2023
Last Modified: August 09, 2023
Protocol Integer ID: 81385
Keywords: RNA, QC, ThermoFisher, UMN
Funders Acknowledgements:
NIH
Grant ID: 1U54AG07041-01
NIH
Grant ID: 1U54AG079754-01
Abstract
RNA quality evaluation of OCT (Optimal cutting temperature) or fresh frozen blocks/samples.
Completed prior to going through -omics and spatial technologies, sc/snRNAseq if applicable.
Purelink RNA Mini Kit Manual
Purelink RNA Mini Kit Manual
Download purelink_rna_mini_kit_man -- Reduced to pages of interest.pdfpurelink_rna_mini_kit_man -- Reduced to pages of interest.pdf

Complete:
Nanodrop for RNA quantification
RIN score for (Eukaryotic total) RNA qualification