Jun 06, 2024
  • Menglin WANG1
  • 1UC Berkeley
  • California Insect Barcoding Initiatives
  • Reagents
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Protocol CitationMenglin WANG 2024. Fresh 80% EtOH. protocols.io https://dx.doi.org/10.17504/protocols.io.5qpvokkz9l4o/v1
License: This is an open access  protocol  distributed under the terms of the  Creative Commons Attribution License,  which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
Protocol status: Working
We use this protocol and it's working
Created: June 05, 2024
Last Modified: June 06, 2024
Protocol  Integer ID: 101288
Keywords: fresh, molecular level, ethanol
Funders Acknowledgements:
Essig Museum of Entomology Research
Grant ID: XXXXXX
Abstract
To make molecular level fresh 80% ethanol.
Protocol materials
UltraPure Distilled Water Invitrogen - Thermo FisherCatalog #10977-015
200 Proof Pure EthanolKOPTECCatalog #V1016
80% EtOH
Use a 50ml Falcon tube to mix 10 mL UltraPure Distilled Water Invitrogen - Thermo FisherCatalog #10977-015 and 40 mL 200 Proof Pure EthanolKOPTECCatalog #V1016 .