May 15, 2024
Version 2
Fresh 4% Paraformaldehyde in PBS V.2
- Allen Institute for Brain Science1
- 1Allen Institute
- Allen Institute for Brain ScienceTech. support
Protocol Citation: Allen Institute for Brain Science 2024. Fresh 4% Paraformaldehyde in PBS. protocols.io https://dx.doi.org/10.17504/protocols.io.x54v9mwnmg3e/v2Version created by Allen Institute
License: This is an open access protocol distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
Protocol status: Working
We use this protocol and it's working
Created: May 15, 2024
Last Modified: May 15, 2024
Protocol Integer ID: 99868
Keywords: RP0196, fixation, perfusion, solution, mouse, mouse brains during the transcardial perfusion process, transcardial perfusion, transcardial perfusion process, preparation, developing mouse brain, immersion fixation,
Abstract
Freshly prepared 4% Paraformaldehyde in PBS is used to fix adult and developing mouse brains during the transcardial perfusion process, as well as for immersion fixation. This solution can be used for no more than one week post-preparation date.
Note: Research reported in this publication was supported by the National Institute Of Mental Health of the National Institutes of Health under Award Number U19MH114830. The content is solely the responsibility of the authors and does not necessarily represent the official views of the National Institutes of Health.
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