Sep 19, 2025
Fluorescent Image Acquisition on VS200
- Allen Institute1
- 1Allen Institute
- Allen Institute for Brain ScienceTech. support
Protocol Citation: Allen Institute 2025. Fluorescent Image Acquisition on VS200. protocols.io https://dx.doi.org/10.17504/protocols.io.n92ld67rog5b/v1
License: This is an open access protocol distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
Protocol status: Working
We use this protocol and it's working
Created: September 19, 2025
Last Modified: September 19, 2025
Protocol Integer ID: 227735
Keywords: fluorescent imaging, Viral SOPs, fluorescent image acquisition on vs200, fluorescent image acquisition, scanning microscope for fluorescent imaging, scanning microscope, proprietary olyvia image, specific exposure times for the viral genetic tool, fluorescent imaging, use of the olympus, image capture, olympus, viral genetic tool, software for image capture, slide, automated slide, glass slide, viewing software, specific exposure time, image, vs200, primate tissues section
Abstract
This protocol details the use of the Olympus (Evident Scientific) VS200 automated slide-scanning microscope for fluorescent imaging of fixed mouse, human, and nonhuman-primate tissues sections mounted on barcoded glass 1x3” or 2x3” slides. The instrument is capable of loading up to 210 1x3” glass slides, or 105 2x3” slides. The images are viewable in Evident’s proprietary OlyVIA image viewing software. This document is intended to describe routine use of the instrument and software, including the initial steps required to configure the software for image capture (section 8.3), which includes specific exposure times for the Viral Genetic Tools (VGT) primary screen.
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