May 13, 2020
Find proteins of unknown function (PUF) using Plantannot - Protocol C
- 1Embrapa
Protocol Citation: Marcos Viana, Adhemar Zerlotini, Mauricio Mudadu 2020. Find proteins of unknown function (PUF) using Plantannot - Protocol C . protocols.io https://dx.doi.org/10.17504/protocols.io.bf88jrzw
License: This is an open access protocol distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
Protocol status: In development
We are still developing and optimizing this protocol
Created: May 11, 2020
Last Modified: May 13, 2020
Protocol Integer ID: 36864
Entering application
Entering application
1s
1s
Enter the Plantannot Result's page, with empty filters and text box search: https://www.machado.cnptia.embrapa.br/plantannot/find/?q=
Or you can enter the https://www.machado.cnptia.embrapa.br/plantannot initial page and click on the magnifying glass with the text box empty as well.
1s
Filtering
Filtering
1s
1s
Visualize the "Filters" card on the left of the page from step1:
1s
In the "Organisms" filter, select any organisms (expand the organism's list using the green arrow) or select all by leaving all boxes empty. We will use Oropetium tomaeum as example. Click "apply" to execute the filter:
1s
In the "Feature type" filter, select "polypeptide", and click "apply" to execute the filter:
1s
In the Orthology filter, select "orthology", and click "apply" to execute the filter:
1s
In the "Orthologs_coexpression" filter, select "co-expression", and click "apply" to execute the filter:
1s
In the "Analyses" filter, select both "no diamond matches" and "no interproscan matches", and click "apply" to execute the filter:
1s
Leave the "Biomaterial" and "Treatment" filters empty:
1s
After execution of all filters we will have the following list of filters:
1s
Viewing results
Viewing results
1s
1s
Visualize the "Results" card on the center-right of the screen, we will have the resulting list of Oropetium's PUFs supposedly related to abiotic stress by using orthology and co-expression networks:
1s