Mar 31, 2026

Public workspaceFFPE Sectioning: 10x Visium HD

DOI
https://dx.doi.org/10.17504/protocols.io.36wgqx163lk5/v1
  • Joanna Bi1
  • 1Stanford University
  • Human BioMolecular Atlas Program (HuBMAP) Method Development Community
    Tech. support email: [email protected]
Joanna Bi
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DOI: https://dx.doi.org/10.17504/protocols.io.36wgqx163lk5/v1
Protocol Citation: Joanna Bi 2026. FFPE Sectioning: 10x Visium HD. protocols.io https://dx.doi.org/10.17504/protocols.io.36wgqx163lk5/v1
License: This is an open access protocol distributed under the terms of the Creative Commons Attribution License,  which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
Protocol status: Working
We use this protocol and it's working
Created: March 31, 2026
Last Modified: March 31, 2026
Protocol Integer ID: 314142
Keywords: 10x genomics visium hd assay, 10x visium hd protocol, embedded tissue block, ffpe sectioning, embedded tissue, sectioning formalin
Abstract
Protocol for sectioning formalin-fixed, paraffin-embedded tissue blocks for the 10x Genomics Visium HD assay.
Troubleshooting
Prepare Fisher Superfrost plus slides. See 10X CG000698 for other acceptable slides.
Determine allowable tissue placement area according to attached document. Download CG000548_VisiumCytAssist-AccessoryKit_QuickReferenceCards_A5format_RevF.pdfCG000548_VisiumCytAssist-AccessoryKit_QuickReferenceCards_A5format_RevF.pdf492KB

Section, dry, and store tissue according to attached document (2.2-2.3).Download CG000684_VisiumHDFFPETissuePrepHandbook_RevD.pdfCG000684_VisiumHDFFPETissuePrepHandbook_RevD.pdf52.5MB