Mar 05, 2020
- 1Hainan University
External link: https://doi.org/10.1371/journal.pone.0234435
Protocol Citation: Jing Xu 2020. Ferrous Ion-chelating Activity. protocols.io https://dx.doi.org/10.17504/protocols.io.bda4i2gw
Manuscript citation:
Zhou J, Yang Q, Zhu X, Lin T, Hao D, Xu J (2020) Antioxidant activities of Clerodendrum cyrtophyllum Turcz leaf extracts and their major components. PLoS ONE 15(6): e0234435. doi: 10.1371/journal.pone.0234435
License: This is an open access protocol distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
Protocol status: Working
We use this protocol and it's working
Created: March 05, 2020
Last Modified: March 05, 2020
Protocol Integer ID: 33852
Abstract
The effects of ferrous ion-chelating activity were conducted according to Guo et al.An aliquot of 1 ml extract was added to a solution of 100 µL of FeCl3 (2.0 mM), 3.7 ml of distilled water and 200 µL of ferrozine (5.0 mM). After 20 min, the absorbance was recorded at 562 nm. The inhibition percent and IC50values were calculated.