Aug 03, 2020
FCMPASS - Creating a cytometer database and datasets
- 1Translataional Nanobiology Section, Laboratory of Pathology, Center for Cancer Research, National Cancer Institute, National Institutes of Health
- Translational Nanobiology Section
Protocol Citation: Joshua A Welsh, Jennifer Jones 2020. FCMPASS - Creating a cytometer database and datasets. protocols.io https://dx.doi.org/10.17504/protocols.io.bhvwj67e
Manuscript citation:
Welsh J A, Jones J C,Small Particle Fluorescence and Light Scatter Calibration Using FCMPASSSoftware,Current Protocols in Cytometry, 94, e79. doi: 10.1002/cpcy.79
License: This is an open access protocol distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
Protocol status: Working
We use this protocol and it's working
Created: June 25, 2020
Last Modified: June 29, 2023
Protocol Integer ID: 38550
Keywords: fcmpass, flow cytometry, calibration, EVs,
Disclaimer
This protocol summarizes key steps for a specific type of assay, which is one of a collection of assays used for EV analysis in the NCI Translational Nanobiology Section at the time of submission of this protocol. Appropriate use of this protocol requires careful, cohesive integration with other methods for EV production, isolation, and characterization. By using the FCMPASS software you agree to the following terms and conditions.
Terms & Conditions of use for FCMPASS software.
Definitions: The term “SOFTWARE” throughout this agreement means the machine readable, binary, object code form, and the related documentation for FCMPASS, a software package that is designed to allow flow cytometer calibration for small particles. The term “RECIPIENT” means the party that downloads the software. The term “PROVIDER” means the National Cancer Institute (NCI), a participating institute of the National Institutes of Health (NIH), and an agency of the United States Government.By downloading or otherwise receiving the SOFTWARE, RECIPIENT may use the SOFTWARE subject to RECIPIENT’s agreement to the following terms:
- THE SOFTWARE SHALL NOT BE USED IN THE TREATMENT OR DIAGNOSIS OF HUMAN SUBJECTS. RECIPIENT is responsible for compliance with all laws and regulations applicable to the use of the SOFTWARE.
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Abstract
This protocol outlines the steps required to create flow cytometer databases and datasets using the FCMPASS software. This is one of a number of protocols in the pipeline for performing small particle calibration using the fcmpass software package.
Materials
FCMPASS software can be accessed at https://nanopass.ccr.cancer.gov.
Open FCMPASS.
Software
FCMPASS
NAME
Click the ‘+’ icon next to ‘Cytometer IDs’ list and enter a unique name to identify a instrument.
Select the relevant cytometer ID to add the dataset to
Click the ‘+’ icon next to the ‘Datasets’ list.
In the window enter the acquisition date of the calibration data and the dataset/experiment name. If there are any notes related to the experiment that are beneficial, they can be entered in the ‘Dataset Notes’ field.