Mar 06, 2020
- 1Hainan University
External link: https://doi.org/10.1371/journal.pone.0234435
Protocol Citation: Jing Xu 2020. Extraction Procedure. protocols.io https://dx.doi.org/10.17504/protocols.io.bdawi2fe
Manuscript citation:
Zhou J, Yang Q, Zhu X, Lin T, Hao D, Xu J (2020) Antioxidant activities of Clerodendrum cyrtophyllum Turcz leaf extracts and their major components. PLoS ONE 15(6): e0234435. doi: 10.1371/journal.pone.0234435
License: This is an open access protocol distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
Protocol status: Working
We use this protocol and it's working
Created: March 05, 2020
Last Modified: March 06, 2020
Protocol Integer ID: 33846
Abstract
Dried leaves of C. cyrtophyllum (150 g) were weighted and sieved (20 mesh) in an herb disintegrator (118 Swing, Zhejiang, China); the powdered samples were extracted twice according to previous protocol [10].Solvent was removed from the combinedfiltrates and 61.44 g of ECE was obtainedand redissolved in distilled water (500 ml). The solution was partitioned with 3×250 ml petroleum ether(60–90 °C), 3×250 ml dichloromethane, 3×250 ml ethyl acetate and 3×250 ml n-butanol. The resulting extracts were concentrated to yield sub-fractions, PEF, DMF, EAF, BAF andRF, whichwere 065, 5.53, 4.13, 13.28 and 36.97 g, respectively. The samples were stored at 4 °C.