To extract plasmid DNA from Agrobacterium, to confirm the correct plasmid has been taken-up, this protocol allows you to directly perform a mini-prep on transformed Agrobacterium, digest this DNA and visualize it on a gel. This is without the need of rescuing the plasmid in E. coli or genotyping with PCR. This protocol using the commercially available Bioline ISOLATE II Plasmid Mini Kit, designed for use with E. coli. The standard protocol this is modified from is available here: https://www.bioline.com/au/downloads/dl/file/id/1219/isolate_ii_plasmid_mini_kit_protocol.pdf