Dec 22, 2025
Ex vivo electrophysiology
- Asa Mackenzie1
- 1Lund University
Protocol Citation: Asa Mackenzie 2025. Ex vivo electrophysiology. protocols.io https://dx.doi.org/10.17504/protocols.io.5jyl8xn57v2w/v1
License: This is an open access protocol distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
Protocol status: Working
We use this protocol and it's working
Created: December 22, 2025
Last Modified: December 23, 2025
Protocol Integer ID: 235676
Keywords: ASAPCRN, electrophysiology
Funders Acknowledgements:
Aligning Science Across Parkinson's
Grant ID: ASAP-020600
Abstract
Ex vivo electrophysiology in Serra et al. 2022
Troubleshooting
Ex vivo electrophysiology
Briefly, mice (>12 months) were deeply anesthetized with an i.p. injection of ketamine/xylazine (75/10 mg/
kg) mixture and then perfused trans-cardially with ice-cold (0C–4C) modified artificial cerebrospinal fluid (ACSF), equilibrated with 95% O2 and 5% CO2, and containing (in mM): 230 sucrose, 26 NaHCO3, 2.5 KCl, 1.25 NaH2PO4, 0.5 CaCl2, 10 MgSO4 and
10 glucose. (pH7,35).
The brain was quickly removed, glued to the stage of a Leica VT1200S vibratome (Leica, Germany), immersed
in the ice-cold ACSF and sectioned into 300 mm thick parasagittal slices.
Slices containing the LHb, the STN and the VP/EP were then
incubated for 1 h to a standard oxygenated ACSF solution, warmed (35C) containing (in mM unless otherwise stated): 126 NaCl, 26 NaHCO3, 2.5 KCl, 1.25 NaH2PO4, 2 CaCl2, 2 MgSO4, 10 glucose, 1 sodium pyruvate and 4.9 mML-gluthathione reduced. Single slices were then transferred to a recording chamber at room temperature 22C–26C and perfused continuously with oxygenated ACSF.