Jun 17, 2024
Evaluation of the Antibacterial efficacy of Allium Sativum Gel as Intracanal Medicament Versus Calcium Hydroxide Paste Against Enterococcas faccalis in Single Rooted Teeth (A Comparative In-Vitro Study)
- Rania Ahmed Ahmed ELnaa1
- 1MSc student , endodontic department, faculty of dentistry cairo university
Protocol Citation: Rania Ahmed Ahmed ELnaa 2024. Evaluation of the Antibacterial efficacy of Allium Sativum Gel as Intracanal Medicament Versus Calcium Hydroxide Paste Against Enterococcas faccalis in Single Rooted Teeth (A Comparative In-Vitro Study). protocols.io https://dx.doi.org/10.17504/protocols.io.36wgqndp3gk5/v1
License: This is an open access protocol distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
Protocol status: In development
We are still developing and optimizing this protocol
Created: June 13, 2024
Last Modified: June 17, 2024
Protocol Integer ID: 101766
Keywords: intracanal medicament versus calcium hydroxide paste against enterococca, antimicrobial efficiency of garlic extract, root canal preparation, antibacterial efficacy of allium sativum gel, extracted teeth, difference between the antimicrobial efficacy, real time pcr technique after root canal preparation, antimicrobial efficiency, antimicrobial efficacy, single rooted teeth, garlic extract, antibacterial efficacy, application of intracanal medicament, intracanal medication, intervention garlic, intracanal medicament in comparison, single rooted root canal, root canal, inoculation of root canal, enterococcus, bacterial dna extraction method, enterococcus faecalis viability, teeth with single canal, vitro study, intracanal medicament, control calcium, calcium, intracanal medicament placement, allium sativum gel, dna extraction
Abstract
Objectives
The aim of this study is to evaluate the antimicrobial efficiency of garlic extract as an intracanal medicament in comparison to calcium hydroxide paste against Enterococcus faecalis viability developed in single rooted root canals by bacterial DNA extraction method using real time PCR.
Research Question
Will application of intracanal medicament of garlic extract differ from calcium hydroxide paste in reduction of E. Faecalis count when used in single rooted teeth?
Null Hypothesis
There is no difference between the antimicrobial efficacy of garlic extract and calcium hydroxide paste in reduction of E. faecalis count in single rooted teeth.
PICO elements
P: Population
Single rooted human freshly extracted teeth with single canals.
I: Intervention
Garlic extract used as intracanal medication.
C: Control
Calcium hydroxide paste used as intracanal medication.
O: Outcome
Bacterial load reduction determined by using real time PCR technique after root canal preparation.
Time of sampling
Sample 1 (S1): after inoculation of root
canals& before instrumentation.
Sample 2 (S2): after instrumentation.
Sample 3 (S3): after intracanal medicament placement for 1 week.
Sample 4 (S4): after intracanal medicament placement for 2 weeks.
Attachments
