This protocol is provided to synthesize single-stranded cDNA from total RNA samples using the TaqMan® MicroRNA Reverse Transcription Kit and quantification of miRNA using the comparative Cycle threshold (Ct) method. Endogenous controls are used to normalize the expression levels of target miRNAs by correcting differences in the amount of cDNA loaded into the PCR reactions. An appropriate constitutively expressed endogenous control must be selected.