Dec 12, 2023
Establishment of patient-derived enteroids/colonoids from endoscopic biopsies
- Tatiana Karakasheva1
- 1Children's Hospital of Philadelphia, Gastrointestinal Epithelium Modeling Program
- CHOP Gastrointestinal Epithelium Modeling ProgramTech. support email: [email protected]
Protocol Citation: Tatiana Karakasheva 2023. Establishment of patient-derived enteroids/colonoids from endoscopic biopsies. protocols.io https://dx.doi.org/10.17504/protocols.io.14egn3rxql5d/v1
License: This is an open access protocol distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
Protocol status: Working
We use this protocol and it's working
Created: December 12, 2023
Last Modified: December 12, 2023
Protocol Integer ID: 92203
Keywords: colonoid culture from endoscopic biopsy, colonoids from endoscopic biopsy, colonoid culture, derived enteroid, colonoid, endoscopic biopsy, enteroid, murine tissue fragment, biopsy, establishment of patient, human tissue, procedure, full procedure, tissue
Funders Acknowledgements:
Children's Hospital of Philadelphia Research Institute
Disclaimer
DISCLAIMER – FOR INFORMATIONAL PURPOSES ONLY; USE AT YOUR OWN RISK
The protocol content here is for informational purposes only and does not constitute legal, medical, clinical, or safety advice, or otherwise; content added to protocols.io is not peer reviewed and may not have undergone a formal approval of any kind. Information presented in this protocol should not substitute for independent professional judgment, advice, diagnosis, or treatment. Any action you take or refrain from taking using or relying upon the information presented here is strictly at your own risk. You agree that neither the Company nor any of the authors, contributors, administrators, or anyone else associated with protocols.io, can be held responsible for your use of the information contained in or linked to this protocol or any of our Sites/Apps and Services.
Abstract
This protocol describes the full procedure of generating an enteroid or colonoid culture from endoscopic biopsy (either freshly procured or recovered from cryopreservation), was developed for human tissue, but works equally well for murine tissue fragments.
Attachments
Troubleshooting