This protocol can be used :to isolate novel cultures from natural samplesto isolate novel cultures from enriched samplesto purify existing cultures and remove contaminantsto obtain clonal cultures from a unialgal strainReference : Marie, D., Le Gall, F., Edern, R., Gourvil, P. & Vaulot, D. 2017. Improvement of phytoplankton culture isolation using single cell sorting by flow cytometry. J. Phycol. in press.