The EPIK™ miRNA Panel Assays protocol is a two‐step protocol consisting of: AB1Step 1. Reverse transcription with miRNA-specific RT‐oligonucleotides and EPIK™ cDNA synthesis kit 2Step 2. Real-Time PCR using SensiSMART™ Master Mix and amplification primers It is critical for the success of the experiment to follow the protocol carefully, from first‐strand cDNA synthesis to real‐time PCR amplification (approximately 2 hours). However, the procedure can be paused after the first‐strand cDNA synthesis and the undiluted cDNA may be stored at ‐20 °C for up to three days.