Aug 28, 2020

Public workspaceENNOLIFE SARS-CoV-2 Antigen Test Kit Protocol

DOI
dx.doi.org/10.17504/protocols.io.bke3ktgn
  • Hsiao-Chung Tsai1
  • 1General manager, ProtectLife International Biomedical Inc.
  • Coronavirus Method Development Community
  • ProtectLife-AnTaimmu
ch_yu
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DOI: dx.doi.org/10.17504/protocols.io.bke3ktgn
Protocol Citation: Hsiao-Chung Tsai 2020. ENNOLIFE SARS-CoV-2 Antigen Test Kit Protocol. protocols.io https://dx.doi.org/10.17504/protocols.io.bke3ktgn
License: This is an open access protocol distributed under the terms of the Creative Commons Attribution License,  which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
Protocol status: In development
We are still developing and optimizing this protocol
Created: August 28, 2020
Last Modified: August 30, 2020
Protocol Integer ID: 41147
Setup ENNOLIFE Clinical Chemistry Analyzer
Setup ENNOLIFE Clinical Chemistry Analyzer
Make sure ENNOLIFE Clinical Chemistry Analyzer and Notebook was connected according to the operation manual. Double click "ENNOLIFE COVID-19 software" shortcut from the desktop.



Keyin Patient IDs then press “START” to check the connection of Analyzer and Notebook.


Three green light means connection is successful.

Running a nasal swab sample
Running a nasal swab sample
For specimen collection of nasal swabs, follow the CDC Swab Collection Guidelines and swab manufacturers' recmommandations. Use a flocked tapered swab. Tilt patient’s head back 70 degrees. While gently rotating the swab, insert swab less than one inch (about 2 cm) into nostril (until resistance is met at turbinates). Rotate the swab several times against nasal wall and repeat in other nostril using the same swab.
Place and soak the Patient Swab into the Tube R1 (extraction buffer). Rotate and stir up and down the swab for 15 secs then standing for 1 min. Duration00:01:15
Mix Tube R1 for several times by swab and discard the swab in biohazard waste.
Transfer solution Amount100 µL from Tube R1 to the Tube R2.

Mix Tube R2 by a vortex mixer. Shaker1500 rpm, 25°C, 00:00:10


Note
Make sure all beads are suspended well.


Insert Tube R2 into carrier of vortex mixer and mix for 15 min. Shaker1500 rpm, 25°C, 00:15:00

While waiting, open pounch and setup the reagent disc into holder. Install the holder on the Analyzer.



Spin down Tube R2. Centrifigation5000 rpm, 25°C, 00:00:10
Insert Tube R2 into Magnetic Rack and stand for 30 secs. Duration00:00:30



Remove supernantant of Tube R2.
Note
Attention, do not remove any magnetic beads.

Add 1000 uL Wash Buffer to Tube R2. Amount1000 µL Wash buffer
Mix for 10 secs by a vortex mixer. Shaker1500 rpm, 25°C, 00:00:10

Spin down Tube R2 for 10 secs. Centrifigation5000 rpm, 25°C, 00:00:10
Insert Tube R2 into Magnetic Rack and stand for 30 secs. Duration00:00:30



Remove supernantant of Tube R2.

Note
Attention, do not remove any magnetic beads.

Transfer solution Amount100 µL from Tube R3 to the Tube R2.
Mix for 10 secs by a vortex mixer. Shaker1005 rpm, 25°C, 00:00:10
Transfer Amount100 µL from Tube R2 (including beads) into the Reagent Disc.



On the Mainpage of the Analyzer
(1) Select “Measurement”. (2) Select “Swab”. (3) Select “START”.



After 10 mins, software will show the results. Duration00:10:00