Electroporation protocol\nWeinstock paper:Matthew T Weinstock,\u00a0Eric D Hesek,Christopher M Wilson,\u00a0Daniel G GibsonVibrio natriegens as a fast-growing host for molecular biology\nNature Methods volume\u00a013,\u00a0pages\u00a0849\u2013851\u00a0(2016)To prepare before:recovery medium(BHI + v2 salts (204 mM NaCl, 4.2 mM KCl, 23.14mM MgCl2), and 680 mM sucrose) sterile filtration \n* i have a box of aliquots in the freezer and pre heat them before use-A vial of competent cells is retrieved from storage at \u221280 \u00b0C and allowed to thaw on ice.-Plasmid DNA and electrocompetent cells are combined and gently mixed in a chilled 1.5-mL microcentrifuge tube. -The cell\u2013DNA suspension is transferred to a chilledelectroporation cuvette with a 0.1-cm gap size.-Cells are electroporated with 900V (in our Electroportor we cant set other parameters ) \n* in the Weinstock paper they recommend depending on the strain 700-900V, 25 \u03bcF and 200 \u03a9-Cells are immediatelyrecovered in 500 \u03bcL preheated (50\u00b0C) recovery medium and transferred to a 1,5-mL tube. \n * we preheat the media to 50\u00b0C because the recovery media is cooled down by pipetting and up taking the chilled cells from the cold cuvettes-The cells are recovered by incubating at 37 \u00b0C for 1.5h. ( also put the agar plates for preheating in the incubator at 37\u00b0C )-The cells are centrifuged down for one mintute at 3000g. The supernant is then discanted.- the pellet is resuspendet in the leftover oft he media and plated out on warm agar plates containing appropriate antibiotic. \n* for Chloramphenicol 2\u00b5g\/mL ; for Kanamycine 200\u00b5g\/mL; for Carbenicillin 200\u00b5g\/mL-The plates are incubated for several hours or overnight at 37 \u00b0C for colonies to appear.