This is a sample Mobility Shift Protocol (NFκB). Each oligo labeled with IRDye 700 provided by LI-COR® Biosciences for EMSA reactions will have an optimized protocol to measure the protein-DNA interaction. See the specific EMSA oligo pack insert for more information. As an example, the NFκB protein-DNA interaction will be described in this protocol. Please refer to your manual to confirm that this protocol is appropriate for the applications compatible with your Odyssey Imager model.