Apr 10, 2024
Electromyogram recordings for internal capsule stimulations in non-human primates
- Lucy Liang1,
- Elvira Pirondini1,
- Jonathan C Ho1
- 1University of Pittsburgh
Protocol Citation: Lucy Liang, Elvira Pirondini, Jonathan C Ho 2024. Electromyogram recordings for internal capsule stimulations in non-human primates. protocols.io https://dx.doi.org/10.17504/protocols.io.rm7vzjn8xlx1/v1
Manuscript citation:
Liang, L., Zimmermann Rollin, I., Alikaya, A., Ho, J.C., Santini, T., Bostan, A.C., Schwerdt, H.N., Stauffer, W.R., Ibrahim, T.S., Pirondini, E., Schaeffer, D.J., 2024. An open-source MRI compatible frame for multimodal presurgical mapping in macaque and capuchin monkeys. BioRxiv https://doi.org/10.1101/2024.02.17.580767
License: This is an open access protocol distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
Protocol status: Working
We use this protocol and it's working
Created: March 26, 2024
Last Modified: May 31, 2024
Protocol Integer ID: 97398
Keywords: ASAPCRN, EMG, non-human primate, corticospinal tract, internal capsule, stimulation, electromyogram recordings for internal capsule stimulation, emg recordings during an internal capsule stimulator, internal capsule implant surgery with the clinical robot, electromyogram recording, internal capsule stimulation, internal capsule implant surgery, implant location, internal capsule stimulator, source mri compatible frame for multimodal presurgical mapping, emg recording, implant surgery, multimodal presurgical mapping, implant, clinical robot, source mri, mri, macaque, capuchin monkey
Funders Acknowledgements:
Aligning Science Across Parkinson's
Grant ID: ASAP-020519
Abstract
EMG recordings is a common method to confirm implant location. In this protocol, we describe steps to perform EMG recordings during an internal capsule stimulator implant surgery. This procedure maybe performed in both sterile and non-sterile surgeries, but here we only describe the steps for a non-sterile, terminal experiment setup.
For step by step guidelines to plan an internal capsule implant surgery with the clinical robot, ROSA ONE Brain, refer to this protocol: dx.doi.org/10.17504/protocols.io.5jyl82rb7l2w/v1
This protocol is supplementary to the manuscript:
Liang, L., Zimmermann Rollin, I., Alikaya, A., Ho, J.C., Santini, T., Bostan, A.C., Schwerdt, H.N., Stauffer, W.R., Ibrahim, T.S., Pirondini, E., Schaeffer, D.J., 2024. An open-source MRI compatible frame for multimodal presurgical mapping in macaque and capuchin monkeys. BioRxiv https://doi.org/10.1101/2024.02.17.580767
Materials
- Needle EMG electrodes (7mm & 13mm)
RLSND107-2.5 & RLSND110-2.5, Rhythmlink, Columbia, SC
- Ripple Neuro Grapevine or any equivalent recording system with 6+ input channels
Ripple Neuro Trellis software
Blue touchproof Microbox
High-voltage protected headstage
- High Power Isolated Stimulator (AM Systems Model 2100)
- Disposable bipolar stimulation probe
- 3M Vetbond tissue glue
- Surgical stapler
- Tape
- Scalpel
Before start
The animal should be under propofol/fentanyl i.v. (or any other anesthetics that allows for synaptic transmission) anesthesia before any step of this protocol is performed. Consult your veterinarian for details.
This protocols describes only the EMG procedure and assumes there is already an electrode placed within the hand area of the internal capsule of the animal.
Needle electrode implants
Make a small incision on the forearm opposite to the side of the internal capsule (IC) electrode implant.
Locate the flexor carpi radialis and/or any other muscle of interest, stimulate at around 1mA, 1Hz with bipolar probe electrode to confirm muscle.
Place two 13mm needle electrodes inside, but along the belly of the muscle for differential EMG recording.
Apply a few drops of Vetbond tissue glue to help the needles stay in place.
Close the incision with staples and tape the electrode cables to the arm. This will prevent them from being pulled out by accident.
Repeat for upper arm, hand, and face muscles, or any other muscles of interest. Incision and stapling are not necessary for face muscles.
IC stimulation & EMG recording
Stimulate the IC at 1Hz or 2Hz with continuous and biphasic pulses, and in the range of 0.8-4.8mA, depending on the motor threshold of the animal.
If no movement was observed during stimulation, adjust the depth of the IC electrode slightly (+/- 2mm) to get a better activation.
Once movement is observed, bring the stimulation amplitude down to motor threshold, record EMG with Ripple Neuro Trellis (or equivalent, at at least 2k samples per second).
Check that the EMG activation is localized to the hand muscles.