This protocol uses the Lonza 4D nucleofection system. Here I present protocols that reduce the price dramatically of using Lonza 4D equipment. 1. The 16 well strip kits are expensive. These can be washed and re-used. This allows a single strip for hundreds of nucleofection experiments.2. The nucleofection buffers are limited (P1, P2, etc). However we have used home made buffers that effectivly target many primary cell types. We have used this Cas9 RNPs (protein\/RNA) and plasmid delivery. We have used this in T and B cells, cell lines, and more.The homemade buffer used here was originally used and published in:https:\/\/cancerres.aacrjournals.org\/content\/71\/23\/7291. It was called Amaxa V. The actual recipe used in that paper was published in the thesis here: https:\/\/opus4.kobv.de\/opus4-fau\/frontdoor\/index\/index\/docId\/2189Here we show a simple nucleofection of plasmid into K562.